Effect of spiromesifen on oxidative stress in bronchial epithelial cells
DOI:
https://doi.org/10.5281/zenodo.18494709Keywords:
Spiromesifen, Cytotoxicity, Oxidative stressAbstract
This study aims to investigate the cytotoxic and oxidative effects of Spiromesifen, a widely used new generation insecticide, on human bronchial epithelial cells, BEAS-2B. The colony formation test was conducted to assess the impact of spiromesifen on cell viability. The oxidative stress state was assessed by measuring Total Oxidative Stress (TOS), Malondialdehyde (MDA), and Glutathione (GSH) levels at concentrations of 12.5, 25, and 50 μM over time. The results indicated that spiromesifen treatment markedly diminished cell viability in a dose- and time-dependent manner with an IC50 value of 100 μM. Following 72 hours of treatment, a statistically significant elevation (p < 0.05) in TOS and MDA levels, indicative of lipid peroxidation, was seen across all concentrations. Conversely, GSH levels, integral to the cellular antioxidant defence system, were observed to be markedly diminished across all concentrations. This study concludes that spiromesifen displays cytotoxicity in human cells, alters cellular redox equilibrium by producing oxidative stress, and causes irreversible molecular damage through lipid peroxidation.
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